廣州市黃埔區(qū)學(xué)大道攬月路廣州企業(yè)孵化器B座402
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1.3.3 LncRNA與miRNA結(jié)合預(yù)測
長鏈非編碼RNA（Long-noncoding RNA，lncRNA）是一類轉(zhuǎn)錄本長度大于200bp的非編碼RNA，可作為人類基因組中一類重要的調(diào)控分子通過多種方式發(fā)揮其生物學(xué)功能． 近年來的研究表明，lncRNA也可以作為一種競爭性內(nèi)源性RNA（competing endogenous RNA），與miRNA互作結(jié)合，形成ceRNA的競爭機(jī)制
以下是我們對數(shù)據(jù)的整理，通過數(shù)據(jù)庫查詢分類、數(shù)據(jù)庫功能及用途、示例結(jié)合分析、數(shù)據(jù)庫優(yōu)化等這四大項，進(jìn)行闡述和演示數(shù)據(jù)庫的查詢和使用，希望對您的實驗項目有所幫助
數(shù)據(jù)庫查詢分類
① lncRNABase：http://starbase.sysu.edu.cn/mirLncRNA.php
② starbase: http://starbase.sysu.edu.cn/
③ RegRNA2.0：http://regrna2.mbc.nctu.edu.tw/detection.html
數(shù)據(jù)庫功能及用途：
1. lncRNABase
數(shù)據(jù)庫功能：
View the predicted miRNA-lncRNA interactions by scanning lncRNA sequences overlapping with CLIP-Seq peaks for potential microRNA targets (miRanda/mirSVR) and then output the detailed information. Here, we list predicted miRNA-target interactions overlapped with CLIP-Seq data
2. starbase：
數(shù)據(jù)庫功能：
1. starBase is designed for decoding Pan-Cancer and Interaction Networks of lncRNAs, miRNAs, competing endogenous RNAs(ceRNAs), RNA-binding proteins (RBPs) and mRNAs from large-scale CLIP-Seq (HITS-CLIP, PAR-CLIP, iCLIP, CLASH) data and tumor samples (14 cancer types, >6000 samples).
2. starBase is also developed for deciphering Protein-RNA and miRNA-target interactions, such as protein-lncRNA, protein-sncRNA, protein-mRNA, protein-pseudogene, miRNA-lncRNA, miRNA-mRNA, miRNA-circRNA, miRNA-pseudogene, miRNA-sncRNA interactions and ceRNA networks from 108 CLIP-Seq (HITS-CLIP, PAR-CLIP, iCLIP, CLASH) datasets.
3. starBase provides miRFunction and ceRNAFunction web tools to predict the function of ncRNAs (miRNAs, lncRNAs, pseduogenes) and protein-coding genes from the miRNA-mediated (ceRNA) regulatory networks
3. RegRNA2.0：
an integrated web server for identifying functional RNA motifs and sites
示例結(jié)合分析：以H19為例
lncRNABase：
1. 打開主頁面
2. 在“l(fā)ncRNA Gene Symbol”對話框中輸入H19
3. 點擊“search”，查詢即可獲取
